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Introducing the New Mieux!
Cutting edge pumping technology for outstanding stability and
reproducibility
The Mieux System is an innovative system developed for nano-LC
for MS. This system is best suited for proteome, metabolome, and
drug metabolism analyses, as well as for high-separation and high-sensitivity
analysis.
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Gradient pumping exceeding
50 nL/min.
Our unique pumping system can achieve a gradient of 50 nL/min. Direct
pumping without using the splitter ensures less change in the flow
rate under conditions which produce pressure fluctuations (associated
with column exchange), offering stable reproducibility and makes
it unnecessary to adjust the split ratio. |
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 Features |
- Unique pumping system, enabling a gradient of 50 nL/min without
using the splitter
- Pressure monitor enables full-time monitoring of pumping state
- Various programs (auto flash, auto refill, auto drain) enable
the System’s high-speed equilibrium and full-automatic
pumping
- Proprietary software enables full-automatic measurement for
1D to 2D systems
- Combining this system with the Spotter Mieux SP (OFF Line/LCMS)
for MALDI-TOF MS enables spotting to a target plate and micro-fractionation
- Connectable to other types of MS
- Unique nano-spray stage and nano-spray column holder available
(optional)
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| Connectable
to other mass spectrometers |
| Applied Biosystems / MDS CIEX · Micromass · BRUKER
DALTONICS · Thermo Fisher, and others |
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| Analysis
data sample (Mieux+Q-Star Elite, t-BSA 50fmol Coverage 70%) |
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| Nano-spray
stages and nano-spray holders for various MS systems |
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| Nano Spray Stage for LCQ |
Nano Spray Stage for LTQ |
Nano Spray Stage Holder for LTQ, Esquire, Accu TOF |
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Mieux System
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Configuration |
| Mieux-I |
Manual injection system. This system enables
desalting condensation manually using the trap column. |
Gradient pump, nano-injection valve with
actuator, control software, PC, analytical column, trap column |
| Mieux-II |
Auto injection system, which enables gradient
analysis after automatic sample injection and desalting. |
Gradient pump, single pump, auto sampler,
trap column valve, control software, PC, analytical column,
trap column (*) |
| Mieux-III |
Direct injection system, which has been
developed for high-sensitivity detection of phosphoric peptides. |
Gradient pump, single pump, auto sampler
(micro specifications), trap column valve, control software,
PC, phosphoric peptide analytical column, trap column (*) |
| Mieux-IV |
Two-dimensional auto injection system.
This on-line two-dimensional system performs the single dimension
ion exchange in a stepwise manner and two-dimensional reverse-phase
separation and elution based on the gradient set-up. |
Gradient pump, single pump, auto sampler,
trap column valve, ion exchange column valve, control software,
PC, ion exchange column, analytical column, and trap column
(*) |
| Mieux-V |
Two-dimensional auto injection system.
This on-line two-dimensional system performs analysis based
on the gradient set-up in one and two dimensions. |
Gradient pumps ×2, single pumps ×2,
auto sampler, trap column valve, ion exchange column valve ×2,
control software, PC, ion exchange column, analytical column,
trap column (*) |
| * Single pump is installed in "Mieux" |
| • Optional functions available for Phosphoric Peptide
Analysis in each systems of Mieux-I, II, IV, and V. |
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| Various
Columns |
| Various column packing, Nano-LC Trap column, Nano-LC
capillary column, Micro, semi-micro, conventional, preperative isolation
column |
Nano-LC capillary column
| Size number |
Column size |
| 2-* |
0.2mm I.D × *mmL |
| 1.5-* |
0.15mm I.D × *mmL |
| 1-* |
0.1mm I.D × *mmL |
| 1-* 0.75-* |
0.075mm I.D × *mmL |
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Nano-LC trap column
| Size number |
Column size |
| 0.5-1 |
0.5mm I.D × 1mmL |
| 0.8-3 |
0.8mm I.D × 3mmL |
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| * Same price regardless of size Please ask the type
of column packing and size which you require. |
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Mieux Series
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| Mieux |
| Gradient Nano Pump |
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Remarks
Auto injection system : single pump included
Manual injection system : single pump not included (gradient
pump only) |
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| Mieux AS |
| Auto Sampler |
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| Mieux AP |
| Single Nano Pump |
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| Mieux SP |
MALDI Spotter
Nano Micro-Fraction Collector |
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Mieux Spotter System For
MALDI-TOF MS
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Example:
1504 spottings on target plate of Applied Bio 4800 |
| By connecting with Mieux,
this system enables stable spotting at nL level. |
| Continuous dispensing to multiple plates |
- Mixed operation of various mfr’s MALDI plates
and Microplates is possible
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| Simultaneous automatic addition of matrix solution |
- Sample eluate and matrix added simultaneously from
the needle tip by using the dedicated nano pump (Mieux
AP)
- Plate is not damaged by spotting because a non-contact
needle is used
- Spotting of sample solution is possible at 100
nL/min by using a dedicated needle
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| Operation control by dedicated software |
- Integrated control of the SP system together with
the Mieux Nano LC system
- Dispensing time can be set freely, starting from
5 seconds
- Dispensing is possible using an external signal
from the detector
- Automatic dispensing time calculation function
based on the entered analytical time and number of
dispensing steps
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| Configuration |
Mieux SP-I |
Mieux-I system, Mieux SP, Mieux AP |
| Mieux SP-II |
Mieux-II system, Mieux SP, Mieux
AP |
| Mieux SP-III |
Mieux-II system, Mieux SP, Mieux
AP Mieux-III system, Mieux SP, Mieux AP |
| Mieux SP-IV |
Mieux-IV system, Mieux SP, Mieux
AP |
| Mieux SP-V |
Mieux-V system, Mieux SP, Mieux AP |
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| Specifications |
| Nano gradient pump (Mieux) |
| Pumping type |
One high-pressure double syringe pumping
system installed
One high-pressure single syringe pumping system installed |
| Syringe volume |
220uL |
| Operation control |
with PC operating dedicated software |
| Communication |
RS-232C |
| Flow rate range |
High-pressure double syringe pumping type
1 nL/min to 200,000 nL/min in 1 nL step (Gradient 50 nL/min
to 1000 nL/min)
High-pressure single syringe pumping type
1 nL/min to 200,000 nL/min in 1 nL step |
| Working pressure |
0.0 to 20.0MPa |
| Pressure indication |
0.0 to 50.0MPa in the 0.1 MPa step |
| Functions |
Auto flush, auto refill, auto drain |
| Backside terminal |
RS-232C, external program start terminal,
pressure output (2-ch) |
| Power supply |
AC100V to 240V |
| Size |
W300 × H210 × D500mm |
| Power consumption |
Max 200 VA |
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| Pumping type |
High-pressure syringe pumping system |
| Syringe volume |
220uL |
| Operation control |
with PC operating dedicated software |
| Communication |
RS-232C |
| Flow range |
1 nL/min to 100,000 nL/min in 1 nL step |
| Working pressure |
0.0 to 15.0Mpa |
| Pressure indication |
0.0 to 50.0MPa in 0.1 MPa step |
| Functions |
Auto flush, auto refill, auto drain |
| Backside terminal |
RS-232C, external program start terminal,
pressure output |
| Power supply |
AC100V to 240V |
| Size |
W140 × H160 × D320mm |
| Power consumption |
Max 150 VA |
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| Auto injector (Mieux AS) (standard
specifications) |
| Sample injection method |
Full loop, partial loop, micro pickup |
| Sample injection rate |
0.1 to 100uL |
| Sample rack |
48 vial containers, 96 plates (Low,High),
384 (Low) |
| Injection accuracy |
Full loop RSD≦0.3
Partial loop RSD≦0.5%(＞5μL)
Micro pickup RSD≦1.0%(＞5μL)
(*For the micro specifications, contact us separately. ) |
| Operation control |
With PC operating dedicated software or
from the keypad on the main body |
| Communication |
RS-232C |
| Cooling function |
Min 4 deg.C (room temperature -16 deg.
C) |
| Power supply |
AC115V(50/60Hz), AC230V(50/60Hz) |
| Size |
W280 × H400 × D440mm |
| Power consumption |
Max. 250VA |
*Descriptions in the catalog are subject to change without notice. |
| Nano HPLC fraction collector (Mieux
SP) |
| Applicable plate |
MALDI Plate |
Applied Biosystems/MDS SCIEX Max 8 plates
Bruker Daltonics Max 4 plates
Waters/Micromass Max 4 plates |
| Microplate |
96 well plate(Low, High), 384 well plate
(Low) |
| Spotting method |
Contact or non-contact spotting,
Matrix mixed spotting without needle contact
(Note: Matrix fluid feed pump is optional.) |
| Probe |
Two-layer (PTFE and SUS) structure
(Sample is center and the matrix solution eluting from the
outside) |
| Allowable dispensing flow range |
100nL/min. ～ |
| Matrix flow rate range |
100nL/min. ～ |
| Spotting mode |
Continuous mode (continuous spotting every fixed given interval)
Continuous spotting by external pulse signal |
| Needle Cleaning |
Automatic needle cleaning by washing solvent
Waste solution port provided |
| Spotting direction |
Straight, zigzagging |
| Operation control |
PC operating dedicated software |
| Communication |
RS-232C |
| Power supply |
AC100 to AC240 |
| Size |
W500 × H330 × D490mm |
| Weight |
Approx. 39 kg |
(Note: Matrix fluid feed pump is optional.) |
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INDEX of Life Science Instruments
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Copyright(c) 1998 YAMATO SCIENTIFIC CO.,LTD. All rights
reserved For more information, call 0120-405-525(Free dial) E-mail:english-website@yamato-net.co.jp
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